Chromosome Analysis is a technique where the chromosomes from cells are visualized under a microscope during metaphase, the stage during cell division when chromosomes are condensed, to analyze the total number and structure of the chromosomes. Staining with Giemsa dye results in a specific banding pattern of light and dark stripes, known as G-banding, and allows identification of each chromosome.
Flow cytometry is a technique for counting, sorting, and analyzing thousands of microscopic particles per second by passing a suspension past an electronic detection apparatus. Flow cytometry can be used for cell counting, cell sorting, and biomarker detection, as well as measuring total DNA, newly synthesized DNA, and gene expression. This technique allows simultaneous multiparametric analysis of physical and/or chemical characteristics.
FISH is a cytogenetic technique that uses a fluorescently labeled probe to detect the location or absence of a target DNA sequence directly on a fixed slide preparation of chromosomes.
Immunohistochemistry is the detection of expression levels and distribution of antigens (e.g. proteins) in a cell or tissue section (usually frozen or paraffin embedded tissues) using antibodies bound to the target that can be visualized through staining.
ISH is a hybridization technique using a labeled complementary DNA or RNA strand as a probe to bind to a specific DNA or RNA sequence target to localize the target within a chromosome or tissue.
Multiplexed Immunofluorescence (MultiOmyx™) is a hyper-plexed, high resolution, immunofluorescence microscopy system capable of illuminating and integrating signals from multiple biomarkers on a cellular and subcellular level in intact tissue on a single slide.
Nucleic acid microarray analysis combines robotics, chemistry, computer science, and biology to study thousands of genes simultaneously. Thousands of spotted samples, DNA, cDNA, or oligonucleotides, are known as probes (with known identity). Probes are immobilized on a solid support, such as a microscope glass slides, silicon chips, or nylon membranes. Nucleic acid microarrays allow analysis of gene expression and discovery, and mutation analysis of many thousands of genes in a single reaction quickly and efficiently.
PCR is a molecular biology technique where a DNA template sequence is exponentially amplified from a single copy by a thermal cycling process. Through repeated cycles of heating and cooling, the target DNA is replicated using a thermo stable DNA polymerase and DNA primers complementary to the target region.
Clarient Diagnostic Services, Inc. is a leading provider of comprehensive, cancer-diagnostic laboratory services. With our advanced technologies, we are able to provide pathologists and oncologists with more accurate and detailed information enabling better characterization and assessment of cancer, which can result in a more accurate diagnosis and more effective treatment. In addition, Clarient’s pharma services are partnering with pharmaceutical companies to reduce cost while accelerating the oncology drug and companion diagnostic development that can result in better outcomes for patients.
From its state-of-the-art diagnostic laboratory to its Internet-based PATHSiTE™ portal, we provide advanced oncology diagnostic services to pathologists, oncologists, hospitals and biopharmaceutical companies. We are also identifying “companion” diagnostic markers for therapeutics in leukemia/lymphoma, as well as breast, prostate, lung and colon cancers.
Clarient is improving the lives of those affected by cancer by bringing clarity to a complex disease.